How to isolate buffy coat?

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  1. Isolating leukocytes from a buffy coat. Pour blood from 1X buffy coat bag (kept at room temp/RT) into 1X T-150 flask. Work in a primary culture hood.
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    Ratings : 11 %   

      
  2. I'm looking to isolate DNA and eventually RNA from buffy coat. I receive whole blood in purple top vacutainers treated with EDTA. These tubes are centrifuged at 3000
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  3. Research Buffy coat specimens remain viable as a DNA source for highly multiplexed genome-wide genetic tests after long term storage
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  4. I want to isolate PBMCs. What I get as a source from the hospital is a Buffy Coat (what I mean here is a big amount of blood pooled together but without plasma, so
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  5. IUGB Plasma and Buffy Coat Processing :

    how to isolate buffy coat

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  6. The buffy coat is the small white layer between the plasma and the red blood cells. It contains white blood cells, for example leucocytes, which contain DNA.
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  7. pluriBead - simple cell isolation directly from whole blood :

    how to isolate buffy coat

    Ratings : 27 %   

      
  8. Buffy coat :

    how to isolate buffy coat

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  9. Buffy Coat Isolation for Manual Ficoll Methods 0001 :

    how to isolate buffy coat

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  10. Isolation of human mononuclear cells from peripheral blood (Genekam Quick MNC isolater) :

    how to isolate buffy coat

    Ratings : 35 %   

      
  11. PRP Buffy coat.AVI :

    how to isolate buffy coat

    Ratings : 22 %   

      
  12. EPVent2 Sample Processing Procedures - The Buffy (DNA) Coat :

    how to isolate buffy coat

    Ratings : 22 %   

      
  13. Mononuclear Cell Isolation from Whole Blood :

    how to isolate buffy coat

    Ratings : 13 %   

      
  14. PBMC Isolation: Buffy Coat Extraction with Biofloaties Aid :

    how to isolate buffy coat

    Ratings : 11 %   

      
  15. Sepax Processing Cryobanks :

    how to isolate buffy coat

    Ratings : 35 %