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I have to dissolve lyophilized primers and get a solution with concentration 100 mkM. What quantity of water should I use and how to estimate it?
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Ratings : 74 %
Calculating oligo water volume :
Ratings : 43 %
Dissolving PCR primers ordered from IDT. 1. Spin the tubes at top speed for 10 minutes to ensure that there are no lyophilized primers stuck to the cap.
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Ratings : 61 %
Freeze-drying, also known as lyophilisation, lyophilization, or cryodesiccation, is a dehydration process typically used to preserve a perishable material or make the
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Ratings : 55 %
The final concentration of primers in a PCR reaction (...) Dissolve the oligo in 90.2 µl to get 500 pmols/µl (...) pmols/µl [ 100 µM (micromolar) ] solution and then
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Ratings : 13 %
Pick a single yeast colony and transfer to liquid media in test tube. :
Ratings : 13 %
How do you know the conc. of lyophilized primer is 20pmol? Is it mentioned in the document that you attached? To make a stock of desired conc. you need to dissolve
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Ratings : 26 %
general cloning - making dna oligo stocks for PCR from lyophilized samples.3gp :
Ratings : 13 %